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细胞微流体工作站VenaFlux (VenaFlux Platforms)是Cellix公司目前*受客户欢迎的一套系统,提供在活细胞水平上微流体活性筛选的完整解决方案。操作简单,分析效率高,可大大提高药品的开发和筛选效率。
VenaFlux 系统是**个能进行连续流体细胞分析的半自动化微流体分析系统。配合使用Cellix生物芯片和细胞分析软件,VenaFlux 系统可检测粘附到微毛细管上的细胞,微毛细管中覆盖抗体或者培养有内皮细胞,模拟生理流动产生一定的剪切力。
VenaFlux 系统操作简单,可减少耗时耗钱的动物模型实验,获得特异性强,准确性高,重复性好的结果。
VenaFlux 流体细胞分析仪产品特点:
? 广泛使用于各种细胞悬浮液;
? 生物芯片可选择用光学显微镜做细节研究;
? 微流体泵可方便的控制流体剪切力在0.05到20dyne/cm2范围内;
? 在分析中可调整剪切力;
? 流体速度可控制在pL/min到mL/min;
? 可做nL到mL 的样品分析;
? 使用DucoCell 分析软件(DucoCell Analysis Software),可简单快速的调节好显微镜的各项参数;
? 统计分析生成的图表(如:细胞粘附性VS.剪切力)可方便的导入Excel文档;
? 电子机械臂移动生物芯片,清洗细微组件和稀释细胞样品;
? VenaFluxTM 系统的软件同步调节泵,翻译阶段筛选,图像采集,样品移动和流体控制,各项操作界面均是用户友好型,系统先进又易于操作;
细胞微流体工作站 (VenaFlux Platforms)的主要技术参数:
Microscope Axiovert 40:
Contrast option: Brightfield, Phase, Varel.
Objectives: 10x LD A-Plan, 20x LD A-Plan, 32x CP-Achromat, 40x LD A-Plan
Eyepieces: E-PL 10x/20 Br., 10x/20 Br. foc.
Stage: Vena8 biochip compatible stage holder, Eppendorf tubes holder.
Mirus 1.0 Nanopump
Specifications: Shear Stress Range of 0.05–20 dyne/cm2,
Volumetric Flow Rates 100nL–20μL/minute (100μL syringe),
Sample Volume Increments Freely adjustable Linear Velocity Range of 10 μm/s to 10 cm/s;
Flow Direction Reversible;
Sample Volume Aspiration Accuracy ±1% ;
Shear Stress Accuracy ±0.5%;
Sample Volume Aspiration Precision < 1%.
Multichannel: 16-Way manifold allowing individual addressing microchannels of Vena8 biochip.
Microscope Cage incubator:
Capabilities: Temperature control ± 0.10C, CO2 and humidity module.
Options: Temperature monitoring software module, black panels for use in fluorescent microscopy.
Deltapix DP 200 USB 2.0 camera
Specifications: 1.3 Mega Pixels (1,280 x 1,024), Color CDD, 1/2" format, Pixel 5.2 μm x 5.2 μm,
Resolution: 15 FPS @ 1,280 x 1,024 pixels; 24 FPS @ 1,204 x 768 pixels; 37 FPS @ 800 x 600 pixels; 60 FPS @ 640 x 480 pixels. Dynamic Range > 60 dB.
Dell Optiplex 745 PC computer
Configuration: Intel Core2 processor; 1.86 GHz; 2GB RAM; ATI Radeon X1300PRO;
Objective Imaging stage control hardware; 19” LCD monitor.
Windows XP Professional SP2;
DeltaPix Viewer Software;
FlowAssay and DucoCell software preinstalled.
FlowAssay Software.
Capabilities: Execution of preset protocols.
Intuitive stepwise protocol interface with steps hierarchy and status control.
DucoCell software
Specifications: Automatic Counting and Detection;
Morphological parameters analyzed automatically(Area, diameter, form-factor, ellipticity, eccentricity, orientation, perimeter, asymmetry, cell centroid coordinates, elliptical axis);
Cell sorting and filtering;
Ability to analyze sub-populations based on inclusion/exclusion of morphological parameters.
Data may be exported to Excel spreadsheet incorporating automatic graph generation (e.g. % cell adhesion vs. shear stress).
File Formats analyzed*.jpg, *.jpeg, *.tif, *.tiff, *.bmp, *.gif, *.png.
Vena8 biochips pack of 10
Specifications: Minimum Sample Volume 3 μL;
Maximum Sample Volume 100 μL; Shear Stress Range of 0.05– 20 dyne/cm2, steps of 0.05dyne/cm2.
Number of channels per biochip is 8;
Volume of each channel is 0.8μL.
Dead volume at input port is 0.1 μL. Vena8 biochip dimensions are 400 μm (W) x 100 μm (D) x 20 mm(L).
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