Schreiber教授因发明PAM系列调制叶绿素荧光仪而获得首届国际光合作用协会（ISPR）创新奖

主要功能

 * 对水生植物、珊瑚、大型海藻样品，或多孔板上的微藻样品进行荧光成像测量

 * 测量程序包括荧光诱导曲线、快速光曲线、淬灭分析、暗弛豫分析等

 * 自定义感兴趣的区域（AOI），数据无限获取

 * 以**个AOI（微藻）为对照，软件自动计算其它所有AOI（有毒物质处理的微藻）的光合活性受抑制的比率

 * 光合横向异质性的定量分析

 * 荧光图像可导出为JPEG、PNG或TIFF格式

应用领域

微藻、大型海藻、珊瑚、水生植物的生理学研究，特别适于模式种的突变株快速筛选，以及大批量微藻样品的毒理学研究。

成像参数Fo， Fm， F， Ft， Fm'， Fv/Fm， ΔF/Fm'， qL， qP， qN， NPQ， Y(NPQ)， Y(NO)， ETR， Abs， NIR和Red等

* 以上所有参数均可成像

* 吸光系数Abs和新参数qL、Y(NPQ)和Y(NO)的成像是IMAGING-PAM独有的

* 生态毒理学研究中，选一个参考点，可以直接求出其它处理（如农药）的受抑制程度Inh.

IMAGING-PAM应用于水生生物学、海洋学、毒理学等领域的部分文献

1.Császár NBM， Ralph PJ， Frankham R， Berkelmans R， van Oppen MJH: Estimating the Potential for Adaptation of Corals to Climate Warming. PLoS ONE2010， 5(3):e9751. doi:9710.1371/journal.pone.0009751.[IMAGING-PAM]

2.Gao S， Chen X-Y， Yi Q-Q， Wang G-C， Pan G-H， Lin A-P， Peng G: A Strategy for the Proliferation of Ulva prolifera， Main Causative Species of Green Tides， with Formation of Sporangia by Fragmentation. PLoS One 2010， 5(1):e8571. doi:8510.1371/journal.pone.0008571.[IMAGING-PAM]

3.段晓琼， 唐秋菊， 禇胜利， 杜林方: 利用Imaging-PAM研究莱茵衣藻对环境变化的影响. 植物生理学通讯2010， 46(3):263-267.[IMAGING-PAM]

4.Chalifour A， Spear PA， Boily MH， DeBlois C， Giroux I， Dassylva N， Juneau P: Assessment of toxic effects of pesticide extracts on different green algal species by using chlorophyll a fluorescence. Toxicological & Environmental Chemistry2009， 91(7):1315-1329.[IMAGING-PAM]

5.Cooper TF， Ulstrup KE: Mesoscale variation in the photophysiology of the reef building coral Pocillopora damicornis along an environmental gradient Estuarine， Coastal and Shelf Science2009， 83(2):186-196.[IMAGING-PAM]

6.Escher BI， Bramaz N， Lienert J， Neuwoehner J， Straub JO: Mixture toxicity of the antiviral drug Tamiflu® (oseltamivir ethylester) and its active metabolite oseltamivir acid Aquatic Toxicology2009， 96(3):194-202.[IMAGING-PAM]

7.Franz S， Altenburger R， Heilmeier H， Schmitt-Jansen M: What contributes to the sensitivity of microalgae to triclosan? . Aquatic Toxicology2009， 90(2):102-108.[IMAGING-PAM]

8.Holzinger A， Roleda MY， Lütz C: The vegetative arctic freshwater green algaZygnema is insensitive to experimental UV exposure Micron2009， 40(8):931-838.[IMAGING-PAM]

9.Mieog JC， Olsen JL， Berkelmans R， Bleuler-Martinez SA， Willis BL， Oppen MJHv: The Roles and Interactions of Symbiont， Host and Environment in Defining Coral Fitness. PLoS One2009， 4(7):e6364.[IMAGING-PAM]

10.Shaw M， Negri A， Fabricius K， Mueller JF: Predicting water toxicity: Pairing passive sampling with bioassays on the Great Barrier Reef Aquatic Toxicology2009， 95(2):108-116.[IMAGING-PAM]

11.Sussman M， Mieog JC， Doyle J， Victor S， Willis BL， Bourne DG: Vibrio Zinc-Metalloprotease Causes Photoinactivation of Coral Endosymbionts and Coral Tissue Lesions. PLoS ONE2009， 4(2):e4511.[IMAGING-PAM]

12.Vermeirssen ELM， Bramaz N， Hollender J， Singer H， Escher BI: Passive sampling combined with ecotoxicological and chemical analysis of pharmaceuticals and biocides – evaluation of three Chemcatcher™ configurations Water Research2009， 43(4):903-914.[IMAGING-PAM]

13.林阿朋， 王超， 乔洪金， 潘光华， 王广策， 宋厉芸， 孙松， 周百成: 青岛海域漂浮和沉降浒苔的光合作用研究. 科学通报2009， 54(4):294-298.[MICROSCOPY-IMAGING-PAM]

14.Abrego D， Ulstrup KE， Willis BL， van Oppen MJH: Species–specific interactions between algal endosymbionts and coral hosts define their bleaching response to heat and light stress Proc R Soc Lond B2008， 275(1648):2273-2282.[IMAGING-PAM]

15.Ainsworth TD， Hoegh-Guldberg O， Heron SF， Skirving WJ， Leggat W: Early cellular changes are indicators of pre-bleaching thermal stress in the coral host Journal of Experimental Marine Biology and Ecology2008， 364(2):63-71.[IMAGING-PAM]

16.Ali NA， Juneau P， Didur O， Perreault F， Popovic R: Effect of dichromate on photosystem II activity in xanthophyll deficient mutants of Chlamydomonas reinhardtii. Photosynthesis Research2008， 95(1):45-53.[IMAGING-PAM]

17.Busch A， Rimbauld B， Naumann B， Rensch S， Hippler M: Ferritin is required for rapid remodeling of the photosynthetic apparatus and minimizes photo-oxidative stress in response to iron availability in Chlamydomonas reinhardtii. Plant Journal2008， 55(2):201-211.[IMAGING-PAM]

18.Escher BI， Bramaz N， Mueller JF， Quayle P， Rutishauser S， Vermeirssen ELM: Toxic equivalent concentrations (TEQs) for baseline toxicity and specific modes of action as a tool to improve interpretation of ecotoxicity testing of environmental samples. Journal of Environmental Monitoring2008， 10:612-621.[IMAGING-PAM， TOXY-PAM]

19.Hall-Spencer JM， Rodolfo-Metalpa R， Martin S， Ransome E， Fine M， Turner SM， Rowley SJ， Tedesco D， Buia M-C: Volcanic carbon dioxide vents show ecosystem effects of ocean acidification. Nature2008， 454:96-99.[IMAGING-PAM， DIVING-PAM]

20.Magnusson M， Heimann K， Negri AP: Comparative effects of herbicides on photosynthesis and growth of tropical estuarine microalgae Marine Pollution Bulletin2008， 56(9):1545-1552.[IMAGING-PAM， TOXY-PAM]

21.Middlebrook R， Hoegh-Guldberg O， Leggat W: The effect of thermal history on the susceptibility of reef-building corals to thermal stress. Journal of Experimental Biology2008， 211(7):1050-1056.[IMAGING-PAM]

22.Muller R， Schreiber U， Escher BI， Quayle P， Nash SMB， Mueller JF: Rapid exposure assessment of PSII herbicides in surface water using a novel chlorophyll a fluorescence imaging assay Science of The Total Environment2008， 401(1-3):1-3.[IMAGING-PAM， TOXY-PAM]

23.Nicolaisen K， Moslavac S， Samborski A， Valdebenito M， Hantke K， Maldener I， Muro-Pastor AM， Flores E， Schleiff E: Alr0397 is an outer membrane transporter for the siderophore schizokinen in Anabaena sp. strain PCC 7120. Journal of Bacteriology2008， 190(22):7500-7507.[IMAGING-PAM]

24.Nielsen HD， Nielsen SL: Evaluation of imaging and conventional PAM as a measure of photosynthesis in thin- and thick-leaved marine macroalgae. Aquatic Biology2008， 3:121-131.[IMAGING-PAM]

25.Roff G， Kvennefors ECE， Ulstrup KE， Fine M， Hoegh-Guldberg O: Coral disease physiology: the impact of Acroporid white syndrome on Symbiodinium Coral Reefs2008， 27(2):373-377.[IMAGING-PAM]

26.Roff G， Ulstrup KE， Fine M， Ralph PJ， Hoegh-Guldberg O: Spatial heterogeneity of photosynthetic activity within diseased corals from the Great Barrier Reef. Journal of Phycology2008， 44(2):526-538.[IMAGING-PAM]

27.Shaw CM， Lam PKS， Mueller JF: Photosystem II herbicide pollution in Hong Kong and its potential photosynthetic effects on corals Marine Pollution Bulletin2008， 57(6-12):473-478.[IMAGING-PAM]

28.Costanzo SD， Watkinson AJ， Murby EJ， Kolpin DW， Sandstrom MW: Is there a risk associated with the insect repellent DEET (N，N-diethyl-m-toluamide) commonly found in aquatic environments? . Science of The Total Environment2007， 384(1-3):214-220.[IMAGING-PAM]

29.Küster A， Altenburger R: Development and validation of a new fluorescence-based bioassay for aquatic macrophyte species Chemosphere2007， 67(1):194-201.[IMAGING-PAM]

30.Runcie JW， Riddle MJ: Assessing the toxic effects of dissolved copper on the Antarctic macroalga Desmarestia menziesii using chlorophyll fluorescence imaging in ecotoxicological tests Toxicological & Environmental Chemistry2007， 89(4):641-653.[DIVING-PAM， IMAGING-PAM]

31.Schreiber U， Quayle P， Schmidt S， Escher BI， Mueller JF: Methodology and evaluation of a highly sensitive algae toxicity test based on multiwell chlorophyll fluorescence imaging. Biosensors and Bioelectronics2007， 22(11):2554-2563.[IMAGING-PAM]

32.Ulstrup KE， Kühl M， Bourne DG: Zooxanthellae harvested by ciliates associated with brown band syndrome of corals remain photosynthetically competent. Applied and Environmental Microbiology2007， 73(6):1968-1975.[IMAGING-PAM]

33.Eggert A， Haubner N， Klausch S， Karsten U， Schumann R: Quantification of algal biofilms colonising building materials: chlorophyll a measured by PAM-fluorometry as a biomass parameter. Biofouling2006， 22(1-2):79-90.[PAM-2000， IMAGING-PAM]

34.Escher BI， Quayle P， Muller R， Schreiber U， Mueller JF: Passive sampling of herbicides combined with effect analysis in algae using a novel high-throughput phytotoxicity assay (Maxi-Imaging-PAM). Journal of Environmental Monitoring2006， 8:456-464.[IMAGING-PAM]

35.Kuster A， Altenburger R: Development and validation of a new fluorescence-based bioassay for aquatic macrophyte species. Chemosphere2006， 67(1):194-201.[IMAGING-PAM]

36.Vopel K， Hawes I: Photosynthetic performance of benthic microbial mats in Lake Hoare， Antarctica. Limnology and Oceanography2006， 51(4):1801-1812.[IMAGING-PAM]

37.Gog L， Berenbaum MR， DeLucia EH， Zangerl AR: Autotoxic effects of essential oils on photosynthesis in parsley， parsnip， and rough lemon. Chemoecology2005， 15:115-119.[IMAGING-PAM]

38.Kühl M， Chen M， Ralph PJ， Schreiber U， Larkum AWD: A niche for cyanobacteria containing chlorophyll d. Nature2005， 433:820.[IMAGING-PAM]

39.Nielsen HD， Nielsen SL: Photosynthetic responses to Cu2+ exposure are independent of light acclimation and uncoupled from growth inhibition in Fucus serratus (Phaeophyceae) Marine Pollution Bulletin2005， 51(8-12):715-721.[IMAGING-PAM]

40.Podola B， Melkonian M: Selective real-time herbicide monitoring by an array chip biosensor employing diverse microalgae. Journal of Applied Phycology2005， 17(3):261-271.[IMAGING-PAM]

41.Ralph PJ， Macinnis-Ng CMO， Frankart C: Fluorescence imaging application: effect of leaf age on seagrass photokinetics. Aquatic Botany2005， 81:69-84.[IMAGING-PAM]

42.Ralph PJ， Schreiber U， Gademann R， Kühl M， Larkum AWD: Coral photobiology studied with a new imaging pulse amplitude modulated fluorometer. Journal of Phycology2005， 41(2):335-342.[IMAGING-PAM]

43.Hill R， Larkum AWD， Frankart C， Kühl M， Ralph PJ: Loss of functional Photosystem II reaction centres in zooxanthellae of corals exposed to bleaching conditions: using fluorescence rise kinetics. Photosynthesis Research2004， 82:59-72.[IMAGING-PAM]

44.Hill R， Schreiber U， Gademann R， Larkum AWD， Kühl M， Ralph PJ: Spatial heterogeneity of photosynthesis and the effect of temperature-induced bleaching conditions in three species of corals. Marine Biology2004， 144:633-640.[IMAGING-PAM]

45.Podola B， Nowack ECM， Melkonian M: The use of multiple-strain algal sensor chips for the detection and identification of volatile organic compounds. Biosensors and Bioelectronics2004， 19:1253-1260.[IMAGING-PAM]