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HORIBA经济型荧光寿命LRET系统
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技术参数

寿命范围From 150 ps to 3 μs
仪器尺寸15" x 13" x 8.8" (WxDxH)
重量25 lbs
灵敏度400 pM fluorescein or better
激发光源OBB proprietary pulsed nanosecond LEDs
脉冲宽度1.5 ns (typical)
激发光谱范围280–670 nm (LED dependent)
发射光谱范围200–650 nm (optional to 900 nm)
激发波长选择By filters
探测器OBB patented lifetime detector
采集时间20 s (sample dependent)
Timescale (menu selectable)Linear, arithmetic and logarithmic
采集模式Fluorescence Decay or Lifetime Kinetics
样品仓Single 1 x 1 cm cuvette (micro-cuvettes available)
软件EasyLife™ X
寿命分析Complete package: 1-to-4 exp, global, non-exponential, micelle kinetics, lifetime distribution (ESM, MEM), anisotropy, FRET calculator
QuickStart DVDIncluded

脉冲LED光源

Nothing could be easier. OBB engineers have developed a broad range of simple, inexpensive, nanosecond pulsed light emitting diodes (LED's) to be used as excitation sources with the EasyLife™ systems. These small but robust light sources are available in a broad range of wavelengths, from UV to NIR. They have excellent pulse to pulse, and long term, stability in their intensity as well as their temporal pulse profiles as is evidenced by the high quality of data the system produces. Switching from one LED to another requires no alignment, and takes only a few seconds. You could not possibly work with any simpler, maintenance-free, light source for fluorescence lifetime research.

脉冲LED的优势

The EasyLife™ pulsed LEDs have certain distinct advantages over more conventional light sources, such as nanosecond flash lamps and lasers.

These advantages are:

  • Inexpensive

  • Durable

  • Require no maintenance

  • Very stable pulse sources

  • Fast

  • Wavelengths from UV to NIR (currently 280–670 nm)

  • Small size

  • Easily interchangeable (snap in—snap out)

Impressive Pulse Stability

6 Hours Repeat Acquisition

Repeat After 4 Days

Click on images to enlarge.

Available LEDs

What you need to do is to select a source or sources. The following list shows you the current choices. To exchange sources is real easy, just snap off and then snap on and it is done—no alignment required.

Below 280 nm we are limited by the current state of the technology for LED’s. Do not despair the future should offer even more choices.

LED Sources:

Central Wavelength

EL 265

excitation LED: 266 +\- 10 nm

EL 278

excitation LED: 280 +\- 10 nm

EL 295

excitation LED: 297 +\- 10 nm

EL 310

excitation LED: 310 +\- 10 nm

EL 340

excitation LED: 340 +\- 10 nm

EL 366

excitation LED: 368 +\- 10 nm

EL 375

excitation LED: 375 +\- 10 nm

EL 405

excitation LED: 403 +\- 10 nm

EL 410

excitation LED: 407 +\- 10 nm

EL 435

excitation LED: 432 +\- 10 nm

EL 445

excitation LED: 444 +\- 10 nm

EL 450

excitation LED: 456 +\- 10 nm

EL 490

excitation LED: 486 +\- 10 nm

EL 505

excitation LED: 510 +\- 10 nm

EL 525

excitation LED: 518 +\- 10 nm

EL 570

excitation LED: 572 +\- 10 nm

LD 630

excitation laser diode: 633 +\- 10 nm

LD 650

excitation laser diode: 649 +\- 10 nm

LD 670

excitation laser diode: 667 +\- 10 nm

Other LED's and laser diodes are available on request.

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